Protein_Domain

Part:BBa_K1092013

Designed by:   Group: iGEM13_Hong_Kong_CUHK   (2013-09-18)

T7-RBS-Voltage Switch

It is a protein domain found in a transmembrane potassium ion channel from Aeropyrum pernix, which is highly voltage dependent. It contain positive charges from the 4 arginine residues, and also numerous leucine residues which give it high hydrophobicity. All these together make it an excellent voltage sensing peptide in the transmembrane region. Study has shown that this helical voltage sensor peptide is capable of moving across the membrane under external voltage, which is the key to the voltage dependence of the ion channel (PMID: 12721618). We make use of this feature to construct a novel voltage switch (BBa_K1092007 & BBa_K1092008), which consist of the PDZ domain (BBa_K1092102), PDZ ligand (BBa_K1092101), this voltage sensor peptide itself. To further adapt this voltage sensor peptide into our project, we added in two (GGGGS)3 flexible linker which serve to link other protein domains, and a (PE)5 linker which serve as a rigid rod that project from the sensor peptide to elongate it and increase the separation distance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None